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NAME: 4-Hydroxyisoleucine [Trigonella foenum-graecum seed extract]

4-Hydroxyisoleucine is a non protein amino acid, isolated from Fenugreek seeds (Trigonella foenum-graecum). Fenugreek has a long history of use in the traditional Indian (Ayurveda) and Chinese medicines. It is a potential product as anti-diabetic, anti-dyslipidemic, muscle building, athletic diet and for skin and scalp health care applications.

Molecular Formula: C6H13NO3
Molecular weight: 147.17232 g/mol

Anti – diabetic activity:

4-Hydroxyisoleucine was demonstrated to stimulate glucose – dependent insulin by direct effect on pancreatic islets (Sauvaire insulin stimulating properties of 4-Hydroxyisoleucine are quite different from those of sulfonylureas. It acts on the pancreatic cells and stimulate the insulin secretion only when the glucose secretion increases above the norm. (Hyperglycemia) and also improves insulin sensitivity. It is a potential therapeutic agent for diabetics (Broca, 2004).

Anti – dyslypidemic activity:

4-Hydroxyisoleucine has also shown significant anti-dyslipidemic activity by decreasing the levels of plasma triglycerides, total cholesterol and free fatty acids in a dyslipidemic hamster model (Narender, 2006).

Muscle building:

Branched chain amino acids, isoleucine, leucine and valine are important to muscle growth and 4-Hydroxyisoleucine, being a branched chain amino acid, is likely to play a similar role. The combination of 4-Hydroxyisoleucine with other amino acids could exert unique physiological responses such as increase in gut absorption of glucose, stimulation of pancreatic beta cells, and enhanced disposal of other carbohydrates (Lee, 2010). 4-Hydroxyisoleucine has a nutrient partitioning effect, which means it may help to shuttle nutrients to muscle cells preferentially over fat cells.


Product Name 4-Hydroxyisoleucine 40%
Botanical Name Trigonella foenum-graecum
Plant part Seeds
Standardization 4-HIL [4-Hydroxyisoleucine]
S. No. Parameters Specification Method
1 Description Off white to gray to Brown colored Visual
2 Identification To comply by HPLC KVN/QC/STP-IN/029
3 Loss on drying NMT 5.0% USP <731>
4 Residue on Ignition NMT 6.0% USP <281>
5 Tapped bulk density 0.30 -0.80g/ml USP <616>
Sieve test (passes through)
# 20 mesh NLT 100.0%w/w
#40 mesh NLT 90.0% w/w
USP <786>
7 Assay by HPLC

NLT 40.0% w/w on dry basis

Heavy Metals
Lead NMT 5.0ppm ICP-OES
Arsenic NMT 1.0ppm ICP-OES
Cadmium NMT 0.3ppm ICP-OES
Mercury NMT 0.1ppm ICP-OES
9 Residual solvents Should comply by ICH/USP USP <467>
Microbiological Profile
Total plate count NNMT 3000cfu/g USP <2021>
Yeast and molds count NMT 100cfu/g USP <2021>
Escherichia coli Absent /10g USP <2022>
Salmonella Absent /10g USP <2022>
Staphylococcus aureus Absent /10g USP <2022>
Pseudomonas aeruginosa Absent /10g USP <62>